bilirubin

From Aaushi
Jump to navigation Jump to search

Physiology

,Bilirubin is a waste product of heme metabolism, produced largely in the spleen where senescent erythrocytes are phagocytosed. However, it is also produced in the brain where it may serve a constituent role as an antioxidant.

Bilirubin is formed from heme by sequential actions of heme oxygease in association with cyt P450 reductase & bilirubin reductase

                    heme oxygenase
                  cyt P450 reductase
heme[Fe+2] + O2   ------------------>   biliverdin + CO + Fe+2
+ NADPH + H+                            + NADP+  + H2O
                biliverdin reductase
    biliverdin  -------------------->   bilirubin + NADP+
  + NADPH + H+

Biliverdin reductase rapidly reduces biliverdin to bilirubin.

Bilirubin binds non-covalently to albumin (unconjugated bilirubin) for transport to the liver, where it is conjugated with glucuronic acid (conjugated bilirubin), then excreted in the bile. In certain conditions, bilirubin may also bind covalently to albumin to form delta-bilirubin.

Bilirubin has the capacity to reduce 10,000 higher fold concentrations of H2O2 through the biliverdin reductase cycle

Laboratory

More general terms

More specific terms

Additional terms

References

  1. Clinical Diagnosis & Management by Laboratory Methods, J.B. Henry (ed), W.B. Saunders Co., Philadelphia, PA. 1991, pg 233
  2. Sedlak TW, Snyder SH. Messenger molecules and cell death: therapeutic implications. JAMA. 2006 Jan 4;295(1):81-9. PMID: https://www.ncbi.nlm.nih.gov/pubmed/16391220

Database