phospholipase B1; membrane-associated; phospholipase B, hPLB; phospholipase B/lipase; PLB/LIP [includes: phospholipase A2; Lysophospholipase] (PLB1, PLB)
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Function
- membrane-associated phospholipase
- Ca+2-independent broad substrate specificity including phospholipase A2/lysophospholipase activity
- preferential hydrolysis at the sn-2 position of diacylphospholipids & diacyglycerol, whereas it shows no positional specificity toward triacylglycerol
- esterase activity toward p-nitrophenyl
- may act on the brush border membrane to facilitate absorption of digested lipids
- undergoes proteolytic cleavage in the ileum (putative)
2-lysophosphatidylcholine + H2O
<-->
glycerophosphocholine + a carboxylate
phosphatidylcholine + H2O
<-->
1-acylglycerophosphocholine + a carboxylate
Inhibition:
- inhibited by diisopropyl fluorophosphate
Structure
- repeat 2 contains the catalytic domain (putative)
- belongs to the 'GDSL' lipolytic enzyme family phospholipase B1 subfamily
Compartment
- apical cell membrane
- present in the intestinal brush border membranes (putative)
Alternative splicing
named isoforms=5
Expression
- expressed in the epidermis (at protein level)