Carboxylesterase 3 (liver carboxylesterase 31 homolog, CES3)

Function

• role in the detoxification of xenobiotics & in the activation of ester & amide prodrugs
• low catalytic efficiency for hydrolysis of CPT-11 (7-ethyl-10-[4-(1-piperidino)-1-piperidino]-carbonyloxycamptothecin), a prodrug for camptothecin used in cancer therapeutics

carboxylic ester + H2O <--> an alcohol + carboxylate

Kinetic parameters:

• KM=137 uM for CPT-11
• KM=460 uM for 7-ethyl-10-[4-(1-piperidino)-1-amino]-carbonyloxycamptothecin

Structure

• belongs to the type-B carboxylesterase/lipase family
• N-glycosylated

Compartment

endoplasmic reticulum lumen (putative)

Expression

expressed in liver, colon & small intestine

More general terms

• glycoprotein
• carboxylase (carboxylic ester hydrolase)

Database

• OMIM: https://mirror.omim.org/entry/605279
• UniProt: http://www.uniprot.org/uniprot/Q6UWW8.html