Acyl-coenzyme A thioesterase 2, mitochondrial; acyl-CoA thioesterase 2; acyl-coenzyme A thioester hydrolase 2a; long-chain acyl-coA thioesterase 2 (ACOT2, PTE2, PTE2A, ZAP128, CTE-Ia)

Function

• acyl CoA thiolesterase

palmitoyl-CoA + H(2)O <--> CoA + palmitate

Kinetic parameters:

• KM=40.3 uM for C10-acyl-CoA
• KM=8.9 uM for C12-acyl-CoA
• KM=1.6 uM for C14-acyl-CoA
• KM=2.0 uM for C16-acyl-CoA
• KM=2.8 uM for C18-acyl-CoA
• KM=4.8 uM for C20-acyl-CoA
• KM=4.5 uM for C16:1-acyl-CoA
• KM=6.1 uM for C18:1-acyl-CoA
• KM=4.3 uM for C18:1-trans-acyl-CoA
• Vmax=212 nmol/min/mg enzyme toward C10-acyl-CoA
• Vmax=681 nmol/min/mg enzyme toward C12-acyl-CoA
• Vmax=766 nmol/min/mg enzyme toward C14-acyl-CoA
• Vmax=656 nmol/min/mg enzyme toward C16-acyl-CoA
• Vmax=488 nmol/min/mg enzyme toward C18-acyl-CoA
• Vmax=408 nmol/min/mg enzyme toward C20-acyl-CoA
• Vmax=661 nmol/min/mg enzyme toward C16:1-acyl-CoA
• Vmax=304 nmol/min/mg enzyme toward C18:1-acyl-CoA
• Vmax=418 nmol/min/mg enzyme toward C18:1-trans-acyl-CoA

Structure

belongs to the C/M/P thioester hydrolase family

Compartment

mitochondrion

Alternative splicing

named isoforms=2

Expression

• strongest expression in heart, liver, muscle & kidney
• weak expression in placenta & pancreas

More general terms

• acyl-coenzyme A thioesterase
• mitochondrial protein

Database

• UniProt: http://www.uniprot.org/uniprot/P49753.html