enzyme linked immunosorbant assay (ELISA)
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Principle
- amplification technique which utilizes an enzyme directly or indirectly linked to an antibody specific for an analyte of interest
- assays are generally run in 96 well microtiter plates
- enzyme which remains in a well is quantified by addition of substrate which reacts to produce a colored product measured absorbance in an automated reader
- used in the context of a competitive binding assay, ELISA may be used as a quantitative assay