restriction fragment length polymorphism (RFLP)

Clinical significance

• polymorphisms may arise from single base mutations, deletions or insertion of DNA
• RFLP analysis is useful for genetic linkage studies to establish carrier (heterozygous) state in kindred of individuals known to be affected with specific genetic disorders

Procedure

• analysis of DNA polymorphisms by agarose gel electrophoresis after cleavage of DNA with restriction endonucleases

Turnaround time: 1-2 days

More general terms

• special chemistry test

Additional terms

• restriction enzyme (endonuclease)