gel filtration

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Principle

  • separation of molecules, especially polypeptides by molecular size by passage of a solution or mixture through columns of beads of cross-linked dextrans or other inert material of defined pore size range
  • the smaller molecules are selectively retained; the larger ones selectively excluded by the column pores
  • thus, the larger molecules elute from the column first, the smaller molecules emerge last

More general terms

References

  1. Stedman's Medical Dictionary 27th ed, Williams & Wilkins, Baltimore, 1999