gel diffusion

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Introduction

Immunochemical method for determining presence of antigen in a specimen. Antigen & antibody are a allowed to diffuse through a semisolid medium such as agar or agarose. When the leading fronts of diffusion of antigen & antibody overlap (& the antigen binds to antibody) formation of a precipitin line will begin. The precipitin line will occur when the antibody is in slight excess.

More general terms

References

  1. ↑ Tietz Textbook of Clinical Chemistry, 2nd ed. Burtis CA & Ashwood ER (eds), WB Saunders Co, Philadelphia PA, 1993, pg 290
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