Sn1-specific diacylglycerol lipase beta (DGL-beta, KCCR13L, DAGLB)

Function

• catalyzes hydrolysis of diacylglycerol to 2-arachidonoyl- glycerol, the most abundant endocannabinoid in tissues
• role in axonal growth during development & for retrograde synaptic signaling at mature synapses

Cofactor: Ca+2

Inhibition:

• inhibited by p-hydroxy-mercuri-benzoate & HgCl2
• inhibited by RHC80267, a drug that blocks 2-AG formation
• not inhibited by PMSF

Kinetic parameters:

• KM=74.1 uM for diacylglycerol;
• Vmax=3.45 nmol/min/mg enzyme
• Optimum pH is 7.0

Structure

• belongs to the AB hydrolase superfamily, lipase family

Compartment

cell membrane

Alternative splicing

named isoforms=3

More general terms

• lipase
• transmembrane 4 protein

Database

• UniProt: http://www.uniprot.org/uniprot/Q8NCG7.html