ubiquitin thioesterase OTUB2; deubiquitinating enzyme OTUB2; OTU domain-containing ubiquitin aldehyde-binding protein 2; otubain-2; ubiquitin-specific-processing protease OTUB2 (OTUB2, C14orf137, OTB2, OTU2)

Function

• hydrolase that can remove conjugated ubiquitin from proteins in vitro & thus may play a role in protein turnover by preventing protein degradation
• mediates deubiquitination of both Lys-48- & Lys-63- linked polyubiquitin chains, with a preference for Lys-63
• thiol-dependent hydrolysis of ester, thioester, amide, peptide & isopeptide bonds formed by the C-terminal Gly of ubiquitin

Structure

• belongs to the peptidase C65 family
• contains 1 OTU domain
• the Asp-48 active site of the catalytic triad imay be located too far to interact directly with the active site His-224, thus OTUB2 may be in an inactive conformation in the absence of ubiquitin & a conformation change may move Asp-48 in the proximity of His-224 in presence of ubiquitin

Alternative splicing

named isoforms=2

Expression

• widely expressed
• expressed at higher level in brain

More general terms

• ubiquitin C-terminal hydrolase

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=78990
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:78990
• OMIM: https://mirror.omim.org/entry/608338
• UniProt: http://www.uniprot.org/uniprot/Q96DC9.html